A study on phenotypic traits of Candida species isolated from blood stream infections and their in vitro susceptibility to fluconazole
نویسندگان
چکیده
Background & objectives: Fungal infections are increasing very rapidly. Candida is a major pathogen involved and constitutes a part of normal microflora of humans. This organism is a leading agent associated with blood stream infections. The present study involves phenotypic characterization of Candida isolates. The isolates were obtained from National Culture Collection of Pathogenic Fungi at Post Graduate Institute of Medical Education and Research, Chandigarh, India. Keeping in view the rising trend of resistance by the Candida species to various antifungal agents their susceptibility has also been studied. Methods: All the isolates were identified upto species level by studying their phenotypic and biochemical characteristics. Phenotypic traits of Candida isolates studied included: formation of pseudohyphae, chlamydospore production, germ tube production, fermentation and assimilation of various sugars by the different species of Candida. All the isolates were tested for their susceptibility to fluconazole by disc diffusion and micro broth dilution methods. Results: The isolates could be clearly differentiated from each other by their phenotypic traits studied. A total of 51 out of 67 isolates examined (76.11%) were sensitive to fluconazole, 6 were sensitive dose dependent and 10 exhibited resistance to this drug. Among the resistant species C. albicans figured on the top followed by C. tropicalis, C. guillermondii and C. glabrata. However, resistance to fluconazole was not observed by C.parapsilosis isolates. Conclusions: The present study would help in the diagnosis of Candida species from clinical samples and give clue about the susceptibility of different species of Candida to fluconazole which might helpin the management of candidal infections.
منابع مشابه
Candidiasis in Pediatrics; Identification and In vitro Antifungal Susceptibility of the Clinical Isolates
Background Candida species are normal microflora of oral cavity, vagina, and gastrointestinal tract. They are the third most prevalent cause of pediatric health care–associated bloodstream fungal infection. This study aimed to provide an epidemiological feature of candidiasis and also presents an antifungal susceptibility profile of clinical Candida iso...
متن کاملMolecular identification and antifungal susceptibility of Candida albicans isolated from Vulvovaginitis Candidiasis
Vulvovaginal candidiasis is caused by the increasing number of Candida species as normal flora in the vagina. To assess the transmission rout as well as to determine the suitable antifungal drugs for treatment, the exact identification of Candida species is crucial. Therefore, earlier detection of infection allows quick initiation of antifungal therapy with a greater probability for improved su...
متن کاملIn vitro activity of econazole in comparison with three common antifungal agents against clinical Candida strains isolated from superficial infections
Background and Purpose: Candida species are the most common organisms involved in superficial fungal infections, worldwide. Although econazole is among the most frequently used topical formulations for the treatment of candidiasis, no information is available regarding the susceptibility profiles of Candida species in Iran. Materials and Methods: In vitro susceptibility of 100 clinical Candida...
متن کاملالگوی حساسیت دارویی گونههای کاندیدا در پوست نوزادان نسبت به فلوکونازول، آمفوتریسین B و کاسپوفانژین
Background: Candida species are opportunistic yeasts that are capable of causing different infections and diseases among immunocompromised patients. Since Candida infections are major causes and frequent of septicemia in Neonatal Intensive Care Units (NICU), and they are associated with high morbidity and mortality rates, so obtaining adequate treatment seems necessary. Low birth weight preterm...
متن کاملCandida Species Isolated from Various Clinical Samples and Their Susceptibility Patterns to Antifungals
Candida is an asexual, diploid, dimorphic fungus that is present on human body and his environment. Nowadays the number of patients, who are immunocompromised, aged, receiving prolonged antibacterial and aggressive cancer chemotherapy or undergoing invasive surgical procedures and organ transplantation, is on increase, and therefore candidiasis emerged itself as an alarming opportunistic dise...
متن کامل